中国胸心血管外科临床杂志

中国胸心血管外科临床杂志

窦性心律与心房颤动患者心房成纤维细胞大电导钙激活钾通道表达差异研究

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目的 通过分析窦性心律与心房颤动患者心房肌成纤维细胞 BKCa 通道表达差异,探索心肌纤维化产生的机制,为治疗和逆转心房颤动结构重构提供新的治疗策略。 方法 选择西南医科大学附属医院心胸外科 2015 年 6 月至 2016 年 12 月收治确诊为风湿性心脏病且行体外循环瓣膜置换术的 10 例患者,其中窦性心律患者(窦性心律组) 5 例,男 2 例、女 3 例,年龄(49.1±8.3)岁;心房颤动患者(心房颤动组) 5 例,男 3 例、女 2 例,年龄(50.3±5.8)岁。于术中获取右心耳组织约 100 mg,采用组织块贴壁法培养心房成纤维细胞,实时荧光定量聚合酶链式反应(qRT-PCR)检测培养的成纤维细胞中 BKCa 通道 mRNA 表达水平及 western blotting 检测 BKCa 通道蛋白的表达情况。 结果 (1)比较心房颤动组及窦性心率组患者的一般情况,两组患者年龄(t=1.21,P=0.67)、性别(t=2.56,P=0.75)差异无统计学意义。两组患者左房内径、右房内径差异有统计学意义(t=19.45,P=0.01;t=23.52,P=0.06)。(2)两组 BKCa α 和 BKCa β1 的 mRNA 表达差异无统计学意义(t=3.14,P=0.79;t=2.88,P=0.69);(3)两组 BKCa α 和 BKCa β1 的蛋白表达差异均无统计学意义(t=0.55,P=0.31;t=0.73,P=0.46)。 结论 BKCa 通道可能不参与心房颤动的发生及维持,其在心肌纤维化进程中可能发挥着作用。

Objective Through analyzing BKCa channel expression in atrial fibroblasts in patients with sinus rhythm and atrial fibrillation (AF), to explore the mechanism of myocardial fibrosis and provide new therapeutic strategies for the treatment and reversal of AF structure reconstruction. Methods We selected 10 patients of rheumatic heart valvular disease who underwent valve replacement surgery. They were 5 patients with sinus rhythm (a sinus rhythm group, 2 males and 3 females with an average age of 49.1±8.3 years) and 5 with AF (an AF group, 3 males and 2 females with an average age of 50.3±5.8 years). About 100 mg tissue was obtained from the right auricula dextra, and the atrial fibroblasts were cultured by tissue block adherence method, and the expression of BKCa channel genes and proteins in cultured fibroblasts was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting methods. Results (1) The general data of 10 patients between the AF group and the sinus rhythm group were compared. There was no significant difference between the two groups in age (t=1.21, P=0.67) and sex (t=2.56, P=0.75). There was statistical difference in the left atrial diameter and the right atrium diameter between the two groups (t=19.45, P=0.01; t=23.52, P=0.06); (2) the mRNA expression of BKCa subunit was detected by qRT-PCR method, and there was no significant difference in the mRNA expression of BKCa α and BKCa β1 between the two groups (t=3.14, P=0.79; t=2.88, P=0.69); (3) the expression of BKCa protein was detected by western blotting method, and there was no significant difference in the protein expression of BKCa α and BKCa β1 between the two groups (t=0.55, P=0.31; t=0.73, P=0.46). Conclusion BKCa pathway may not be involved in the pathogenesis and maintenance of AF, but it may play an important role in the process of myocardial fibrosis.

关键词: 心房颤动; 窦性心律; 成纤维细胞; BKCa

Key words: Atrial fibrillation; sinus rhythm; fibroblasts; BKCa

引用本文: 毕红亲, 李妙玲, 周伟, 李光, 董亚辉, 黄涛, 于风旭. 窦性心律与心房颤动患者心房成纤维细胞大电导钙激活钾通道表达差异研究. 中国胸心血管外科临床杂志, 2017, 24(12): 937-942. doi: 10.7507/1007-4848.201707042 复制

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